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2022-05-05 # Protocol ID Title Link author name Author email Author affliation Issue Research article title Research article link Journal 4401 Expression, Purification, and in vitro Enzyme Activity Assay of a Recombinant Aldehyde Dehydrogenase from Thermus thermophilus, using an Escherichia coli host httsp://bio-protocol.org/e4401
Kim Shortall kimleah.shortall@ul.ief Department of Chemical Sciences, Bernal Institute, University of Limerick, V94 T9PX, Limerick, Ireland
2022-05-05 "Study of ALDH from Thermus thermophilus-Expression, Purification and Characterisation of the Non-Substrate Specific, Thermophilic Enzyme Displaying Both Dehydrogenase and Esterase Activity") https://pubmed.ncbi.nlm.nih.gov/34944041/ Cells Edmond Magner edmond.magner@ul.ie Tewfik Soulimane tewfik.soulimane@ul.ie 44024 Quantitative Analysis of Actin Cable Length in Yeast httsp://bio-protocol.org/e4402 Shane G. McInally shanemcinally@brandeis.eduC Department of Biology, Brandeis University, Waltham, MA, 02454, USAS Scaling of subcellular actin structures with cell length through decelerated growth# https://doi.org/10.7554/eLife.68424 Elife Jane Kondev kondev@brandeis.eduC Department of Physics, Brandeis University, Waltham, MA, 02454, USA Bruce L. Goode goode@brandeis.edu 4403V Immunoisolation of Endosomal Recycling Vesicles from Saccharomyces cerevisiae httsp://bio-protocol.org/e4403
Sho W. Suzuki Weill Institute for Cell and Molecular Biology and Department of Molecular Biology and Genetics, Cornell University, Ithaca, USAS A PX-BAR protein Mvp1/SNX8 and a dynamin-like GTPase Vps1 drive endosomal recycling) https://pubmed.ncbi.nlm.nih.gov/34524084/ Scott D. Emr sde26@cornell.edu 4404\ A System to Easily Manage Metadata in Biomedical Research Labs Based on Open-source Software httsp://bio-protocol.org/e4404 Manuel A. Castro-Alamancos mcastro@uchc.edub Department of Neuroscience, University of Connecticut School of Medicine, Farmington CT 06001, USAk A signaled locomotor avoidance action is fully represented in the neural activity of the midbrain tegmentum2 https://www.doi.org/10.1523/JNEUROSCI.0027-21.2021
J Neurosci 4406x Production and Crystallization of Nanobodies in Complex with the Receptor Binding Domain of the SARS-CoV-2 Spike Protein httsp://bio-protocol.org/e4406 Audrey Le Bas audrey.lebas@rfi.ac.ukQ Structural Biology, The Rosalind Franklin Institute, Harwell Science Campus, , UKs A potent SARS-CoV-2 neutralising nanobody shows therapeutic efficacy in the Syrian golden hamster model of COVID-19) https://pubmed.ncbi.nlm.nih.gov/34552091/
Nat Commun Halina Mikolajek halina.mikolajek@diamond.ac.uk6 Diamond Light Source Ltd, Harwell Science Campus, , UK
Jiandong Huo jiandong.huo@strubi.ox.ac.uk Chelsea Norman chelsea.norman@rfi.ac.uk Joshua Dormon joshua.dormon@rfi.ac.ukQ Structural Biology, The Rosalind Franklin Institute, Harwell Science Campus, , Uk James H. Naismith james.naismith@strubi.ox.ac.uk Raymond J. Owens ray.owens@strubi.ox.ac.uk 4407e Translating Ribosome Affinity Purification (TRAP) of Cell Type-specific mRNA from Mouse Brain Lysates httsp://bio-protocol.org/e4407 Catherine L. Salussolia* catherine.salussolia@childrens.harvard.edu F . M . K i r b y N e u r o b i o l o g y C e n t e r , R o s a m u n d S t o n e Z a n d e r T r a n s l a t i o n a l N e u r o s c i e n c e C e n t e r , D e p a r t m e n t o f N e u r o l o g y , B o s t o n C h i l d r e n s H o s p i t a l , H a r v a r d M e d i c a l S c h o o l , B o s t o n , M a s s a c h u s e t t s 0 2 1 1 5 , U S A t Loss of Tsc1 in cerebellar Purkinje cells induces transcriptional and translation changes in FMRP target transcripts# https://doi.org/10.7554/eLife.67399 Kellen D. Winden# kellen.winden@childrens.harvard.edu Mustafa Sahin# mustafa.sahin@childrens.harvard.edu 4408d A Molecular Cloning and Sanger Sequencing-based Protocol for Detecting Site-specific DNA Methylation httsp://bio-protocol.org/e4408 Wei Guoc Department of Botany and Plant Pathology, Purdue University, West Lafayette, Indiana, United Statesx RNA-directed DNA methylation prevents rapid and heritable reversal of transposon silencing under heat stress in Zea mays0 https://www.doi.org/10.1371/journal.pgen.1009326
PLoS Genet Anthony Cannon anthcann@indiana.edu Damon Lisch dlisch@purdue.edu 4409H Quantification of Soil-surface Roots in Seedlings and Mature Rice Plants httsp://bio-protocol.org/e4409
Eiko Hanzawa eiko.hanzawa.a5@tohoku.ac.jpS Graduate School of Life Sciences, Tohoku University, 980-8577 Sendai, Miyagi, JapanW Root angle modifications by the DRO1 homolog improve rice yields in saline paddy fields+ http://www.ncbi.nlm.nih.gov/pubmed/32817523 PNAS Yuka Kitomi ykitomi@affrc.go.jpw Institute of Crop Science, National Agriculture and Food Research Organization (NARO), 305-8518 Tsukuba, Ibaraki, Japan Yusaku Uga yuga@naro.affrc.go.jp` , Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba, Japan Tadashi Sato tadashi.sato.d1@tohoku.ac.jpZ Graduate School of Agricultural Science, Tohoku University, 980-8572 Sendai, Miyagi, Japan 4410F Bacterial Growth Curve Measurements with a Multimode Microplate Reader httsp://bio-protocol.org/e4410 Ariel T. Rogers atr007@uark.eduD Department of Physics, University of Arkansas, Fayetteville, AR, USAM A new analysis method for evaluating bacterial growth with microplate readers) https://pubmed.ncbi.nlm.nih.gov/33434196/ PLoS One Kaitlin R. Bullard krcallie@uark.edu Akash C. Dod akashdod@uark.edu Yong Wang yongwang@uark.edu 4411A Ex vivo Human Skin Infection with Herpes Simplex Virus 1 httsp://bio-protocol.org/e4411 Nydia C. De La Cruz nydia.delacruz@uni-koeln.de] Center for Biochemistry, University Hospital Cologne, University of Cologne, Cologne, Germany Ex vivo infection of human skin with herpes simplex virus 1 reveals mechanical wounds as insufficient entry portals via the skin surface) https://pubmed.ncbi.nlm.nih.gov/34379501/ J Virol Maureen Mckel mmoeckel@uni-koeln.de Lisa Wirtz lisa.wirtz@uk-koeln.de Dagmar Knebel-Mrsdorf dagmar.moersdorf@uni-koeln.de H { O j
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